Table 2

Heterodimerization assay; ability of 6His-tagged multiple-alanine mutants to bind untagged, wild-type Arc to Ni-NTA agarose

6His-tagged proteinFraction untagged Arc in
Ni-NTA flow-through + washNi-NTA imidazole elution
3A0.890.11
7A0.780.22
11A0.730.27
12A0.660.34
15A0.560.44
λN-domain-H61.000.00
None0.990.01