Table 1

Percentage of TGR-1 fibroblast nuclei containing ssDNA foci

Treatment% Nuclei with ssDNA foci
Total<10 Foci>10 Foci
None1<1<1
None211
None211
Denaturation931182
Cycloheximide (10 μg/ml) (for 1 hr)211
Actinomycin D (100 ng/ml) (for 1 hr)312
Etoposide (0.5 μg/ml) (for 1 hr)32239
Etoposide (2 μg/ml) (for 1 hr)432914
Etoposide (6 μg/ml) (for 1 hr)573324
Etoposide (20 μg/ml) (for 1 hr)611546
MMC (0.5 μg/ml) (for 1 hr)361224
3 hr after MMC271116
6 hr after MMC331419
15 hr after MMC29524
24 hr after MMC321121
48 hr after MMC13112
1 hr after 0.5 Gy21165
1 hr after 1 Gy362610
1 hr after 2 Gy402020
1 hr after 5 Gy452520
1 hr after 10 Gy491534
6 hr after 1 Gy22139
6 hr after 5 Gy251411
24 hr after 0.5 Gy1037
24 hr after 1 Gy1477
24 hr after 2 Gy1569
24 hr after 5 Gy211011
24 hr after 10 Gy301614
  • At least 300 nuclei were analyzed for each experiment.