Table 2

Induction of Rad51 and ssDNA foci by DNA damage

Cell line treatment% Cells with
No focissDNA foci (only)Rad51 foci (only)ssDNA and Rad51 foci*
TotalCo+
TGR-1
 24 hr after etoposide154253819
PPL
 24 hr after etoposide81946947
 24 hr after 0.5 Gy77621514
 24 hr after 1 Gy581752019
 24 hr after 2 Gy531362827
 24 hr after 4 Gy422822826
 24 hr after 8 Gy193573933
 24 hr after 10 Gy221575643
 6 hr after 10 Gy342653522
 48 hr after 10 Gy35324235
KRA
 24 hr after 10 Gy281894530
XPA
 None62194159
  • At least 300 cells were analyzed for each experiment. 

  • * Co+ cells show >10% colocalization of Rad51 and ssDNA foci.