Table 3

Colocalization frequencies of ssDNA foci with Rad51 and RPA

Probe 1Probe 2PPL fibroblasts 24 hr after 10 Gy [No. (percent) of foci per nucleus stained with] XPA fibroblasts without treatment [No. (percent) of foci per nucleus stained with]
1 and 21 only2 only1 and 21 only2 only
Rad51ssDNA6.8 (35%)5.4 (27%)7.0 (38%)10.7 (44%)5.4 (22%)8.2 (34%)
RPAssDNA7.5 (50%)3.7 (25%)3.8 (25%)9.5 (53%)7.2 (41%)1.1 (6%)
Rad51*CREST*0.7 (3%)10.3 (52%)8.7 (45%)1.0 (4%)10.4 (47%)10.8 (49%)
  • 50 cells with at least 5 foci of each type were analyzed for each double-staining (Rad51 and ssDNA, RPA and ssDNA, Rad51 and CREST) experiment. 

  • * Because Rad51 foci and centromeres don’t interact functionally, Rad51–CREST double-staining foci are caused by chance associations.