Table 1

The recombinant plasmid constructions used in this work

No.ConstructPlasmidCHL D fragmentCloning sites
1GST-CHL D(N)pEGKT 63-374BamHI/HindIII
2GST-CHL D(NM1LM2)pEGKT 63-485BamHI/HindIII
3GST-CHL D(M1LM2)pGEX4T-1375-485BamHI/EcoRI
4GST-CHL D(M1LM2C)pEGKT375-748XbaI/SalI
5CHL DpGEM-7Zf(−) 63-748Xbal/BamHI
6CHL IpGEM-7Zf(−) ChlI XbaI/HindIII
7CHL HpCR II ChlH
8BD-CHL D(N)pAS2-1 63-374EcoRI/BamHI
9BD-CHL D(NM1)pAS2-1 63-398EcoRI/BamHI
10AD-CHL D(NM1)pACT2 63-398BamHI/EcoRI
11BD-CHL D(NM1LM2)pAS2-1 63-485EcoRI/BamHI
12AD-CHL D(NM1LM2)pACT2 63-485BamHI/EcoRI
13BD-CHL D(M1LM2)pAS2-1375-485EcoRI/BamHI
14AD-CHL D(M1LM2)pACT2375-485BamHI/EcoRI
15BD-CHL D(M1LM2)pACT2-1375-748EcoRI/BamHI
16AD-CHL D(M1LM2C)pACT2375-748BamHI/EcoRI
17BD-CHL D(M2C)pAS2-1448-748EcoRI/BamHI
18AD-CHL D(M2C)pACT2448-748BamHI/EcoRI
19BD-CHL D(C)pAS2-1486-748EcoRI/BamHI
20(M1LM2C)CHL D-lexApFBL23375-748EcoRI/BamHI
21BD-CHL I-CHL D(M1LM2C)pAS2-1375-748BamHI/SalI
22CHL Ip423TEF ChlI SmaI/SalI
23AD-CHL DpACT2 63-748SmaI/EcoRI
24AD-CHL IpACT2 ChlI SmaI/XhoI
25AD-CHL HpACT2 ChlH SmaI/XhoI
26BD-CHL DpAS2 63-748SmaI/SalI
27BD-CHL IpAS2 ChlI SmaI/SalI
28BD-CHL HpAS2 ChlH SmaI/SalI