Table 1

Summary of agonist-induced Δ[Zn2+]i and Δ[Ca2+]i

[Ion]i (μM)NMDA + Zn2+, 5 experiments, 162 neurons KCl + Zn2+, 6 experiments, 223 neurons Kainate + Zn2+, 7 experiments Kainate + Ca2+, 5 experiments
Ca-A/K(+), 31 neuronsCa-A/K(−), 345 neuronsCa-A/K(+), 33 neuronsCa-A/K(−), 217 neurons
Peak at 5 min0.04 + 0.003*0.58 ± 0.02 2.3 ± 0.02 0.64 ± 0.03 34.0 ± 3.0 7.8 ± 0.2
Recovery at 60 min<0.01*0.12 ± 0.010.54 ± 0.05 0.21 ± 0.01 <1.5*#<1.5*#
  • For Δ[Zn2+]i measurements, cortical cultures were loaded with Newport green and exposed for 5 min to 300 μM Zn2+ with 100 μM kainate (+10 μM MK-801); with 50 mM K+ (+10 μM MK-801, 10 μM NBQX); or with 100 μM NMDA (+10 μM NBQX), as in Fig. 2. For Δ[Ca2+]i measurements, cultures were loaded with Fura-2FF and exposed for 5 min to 100 μM kainate in the presence of 1.8 mM Ca2+ (+10 μM MK-801), as in Fig. 6. Values in table show mean (±SEM) peak levels (at end of 5-min exposure) and levels 60 min after washout of the agonist, compiled from ≥ experiments. *Indicates that calibrated values are below the threshold for accurate quantification with the indicator. # indicates the complete recovery of [Ca2+]i to basal levels (<150 nM) within 60 min after the exposures in control experiments using the high-affinity indicator fura-2.