Table 3

Time course of mitotic and apoptotic responses in Igf1−/− and wt uterine epithelium

TreatmentMI, % DI, % Cell no. Cell size
wtIgf1−/−PwtIgf1−/−PwtIgf1−/−PwtIgf1−/−P
Vehicle0.87  ± 0.240.22  ± 0.180.10.42  ± 0.220.13  ± 0.080.3264  ± 13247  ± 460.739.2  ± 1.5 μ6.8  ± 0.4 μ0.17
21 h post-E216.03  ± 1.034.64  ± 1.260.00011.21  ± 0.160.50  ± 0.080.44400  ± 49230  ± 190.00316.5  ± 1.2 μ10.5  ± 1.0 μ0.003
48 h post-E21.19  ± 0.184.60  ± 0.720.01580.33  ± 6.32.08  ± 0.750.0001492  ± 78357  ± 280.05517.1  ± 13 μ14.6  ± 1.0 μ0.19
  • The mitotic index (MI) and death index (DI) were obtained by scoring the number of mitotic and apoptotic figures per 100 epithelial nuclei in hemotoxylinleosin-stained uterine sections. Evaluation of mitotic and apoptotic figures by DAPI (4,6-diamidino-2-phenylindole) staining confirmed the findings with hemotoxylinleosin. The cell number data represents the number of luminal epithelial cells present in anatomically matched uterine cross sections. The cell size data represents the length of luminal epithelial cells from apical to basement membranes. All data are mean ± SEM, with 3–12 mice in each group.