Table 2

Sequence analysis of transposon-tagged genes reveals homologies with polysaccharide biosynthesis motifs

Mutant strainHomologous proteinDegree of homology, % identity/ % similarityFunction of the homologous proteinOrganism
38, (2*, 44*, 49*)Ipi125.0/33.8Isopentenyl-diphosphate delta-isomerase Arabidopsis thaliana
3 YwlE37.9/51.7Protein tyrosine phosphatases Bacillus subtilis
65, (8, 41)ExoP22.3/35.2Polymerization and/or export of succinoglycan Rhizobium meliloti
21, (24)EpsD74.5/80.1NDP-N-acetyl-d-galactosaminuronic acid dehydrogenase Ralstonia solanacearum
32, (23, 60)AmsK34.1/43.3Biosynthesis of amylovoran Erwinia amylovora
59 (25*)CysE17.0/21.9Serine acetyl transferase Synechocystis PCCC6803
54, (31, 35, 68)GumD45.0/38.4Glycosyl-1-phosphate transferase Xanthomonas campestris
39 EpsC66.2/69.8UDP-N-acetylglucosamine 2-epimerase R. solanacearum
50 Cap1K16.2/26.5UDP-glucose dehydrogenase Streptococcus pneumoniae
69 ExoF28.9/36.1Biosynthesis of succinoglycan R. meliloti
70 CapK28.9/33.3Biosynthesis of type1 capsular polysacchardie Staphylococcus aureus
74 ORF1125.8/36.6Biosynthesis of serotype K2 capsular polysaccharide Klebsiella pneumoniae
76 WcaL17.9/28.0Glycosyl transferase Escherichia coli
4, 9, 18, 73None
  • Based on the identification of homologous sequences of proteins of known function. Degree of homology represented in the third column corresponds to the numbers shown in bold, in the first column. 

  • * Homologies were determined after identification of overlapping contigs from the V. cholerae O1 tigr sequencing project.