Table 2

Comparisons between leuB reversion rates and leuB mRNA levels in CP78, CP79, and 78AL

CellsConditionleuB mRNA*MR × 10−9
CP79 relAlog29  ± 5Not applicable
CP79 relALeu-starved17  ± 50.22  ±  0.1
CP78 relA+log30  ± 5Not applicable
CP78 relA+Leu-starved269  ± 601.5  ±  0.5
78ALlog213  ± 41.0  ±  0.1
78ALlog + IPTG1,275  ± 175 2.7  ±  0.5
78ALSP − Leu63  ± 40.9  ±  0.1
78ALSP − Leu + IPTG333  ± 321.6  ±  0.5
  • SP, stationary phase. 

  • * pg leuB mRNA/μg total RNA. 

  • leuB mutation rates (MR) ×10−9 (see Materials and Methods). 

  • Lacks attenuation and ppGpp-binding sequences.