Table 1

Staphylococcal surface proteins are required to establish acute infections in mice

S. aureus straincfu injectedMortality, %
Experiment 1
Newman (wt)1.25  × 10890
Newman (wt)1.25  × 10790
Newman (wt)1.25  × 1060
SKM3 (srtA)1.35  × 10820
SKM3 (srtA)1.35  × 1070
SKM3 (srtA)1.35  × 1060
Experiment 2
Newman (wt)7.0  × 107100
Newman (wt)7.0  × 10640
Newman (wt)7.0  × 1050
SKM3 (srtA)1.1  × 10840
SKM3 (srtA)1.1  × 1070
SKM3 (srtA)1.1  × 1060
  • S. aureus strains Newman (wild-type) and the isogenic sortase mutant SKM3 were grown overnight in TSB supplemented with 5% sheep's blood at 37°C. The cultures were diluted 1∶10 in fresh medium and incubated with shaking for 5 h. Staphylococci were diluted into 5% hog gastric mucin, and 0.5 ml was injected i.p. into 10 CD1 mice per dilution. Animals were observed for 72 h, and moribund mice were euthanized. Average LD50 Newman 7.2 × 106 (Exp. 1), 7.58 × 106 (Exp. 2) and SKM3 1.8 × 108 (Exp. 1), 1.2 × 108 (Exp. 2) (P < 0.05).