Table 2

Targeting genes for replacement by the cat cassette

Target Site*42°C, minCmR Recombinants
galK 0<1
galK 152.5 × 104
cIII kil gam 0<1
cIII kil gam 155.0 × 104
  • * DY330 competent cells were electroporated with 100 ng of the cat cassette targeted to replace either galK (galK<>cat) or prophage genes cIII kil gam (cIII kil gam<>cat). 

  • Total recombinants per electroporation are shown.