Table 3

Genetic requirements on λ

Strain*ProphageRecombinant
DY330wild-type4,100
DY392(hin-int)<>amp2,000
DY351(sieB-kil)<>cat4,400
DY386(hin-int)<>amp1,650
(sieB-kil)<>cat
DY349(gam)<>cat0
DY360(bet)<>cat0
DY359(exo)<>cat0
  • * Competent cells were induced for 15 min and electroporated with 10 ng of linear galK<>tet

  • Parenthesis indicate deleted prophage genes (Fig. 1). 

  • Total recombinants per electroporation are shown.