Table 2

Generation of reassortant influenza A viruses between A/Teal/HK/W312 (H6N1) and A/WSN/33 (H1N1) by cotransfecting eight plasmids

Segment*HANAMNSHA-NA-M-NS
1pHW241-PB2pHW241-PB2pHW241-PB2pHW241-PB2pHW241-PB2
2pHW242-PB1pHW242-PB1pHW242-PB1pHW242-PB1pHW242-PB1
3pHW243-PApHW243-PApHW243-PApHW243-PApHW243-PA
5pHW245-NPpHW245-NPpHW245-NPpHW245-NPpHW245-NP
4 pHW184-HA pHW244-HApHW244-HApHW244-HA pHW184-HA
6pHW246-NA pHW186-NA pHW246-NApHW246-NA pHW186-NA
7pHW247-MpHW247-M pHW187-M pHW247-M pHW187-M
8pHW248-NSpHW248-NSpHW248-NS pHW188-NS pHW188-NS
Virus titer2 × 1022 × 1032 × 1052 × 1074 × 106
  • * Plasmids containing the cDNA of A/WSN/33 (H1N1) are shown in bold. 

  • The numbers represent infectious virus particles per milliliter of supernatant of transfected cells as determined 72 h after transfection.