Table 2

Intracellular amounts of PMEA, PMEAp, and PMEApp (in pmol per 106 cells) in cells incubated with bis-POM-[3H]PMEA

Cell linePMEA, pmol PMEAp, pmol PMEApp, pmol Total, pmol
0 h1 h2 h0 h1 h2 h0 h1 h2 h0 h1 h2 h
293138104750.73.26.20.415.227.6139122109
293/C138111810.53.56.20.412.522.3139127109
293/MRP5E13681390.52.44.20.311.816.11379560
293/MRP5I12861190.53.03.40.311.412.61287635
  • Samples containing PMEA were separated by HPLC for analysis of PMEA, PMEAp, and PMEApp as described (33, 34). Total, total PMEA equivalents.