Table 1

Mutations contributing to the reverse phenotype of tTA

TransactivatorAmino acid exchanges
rtTAEK71DN95LS101GD102
rtTA-S2EG19AP56DE148HR179
rtTA-S3TA26DG95
rtTA-19/56REG19AP56
tTA-148/179RDE148HR179
rtTA-M1SG12EG19AP56
rtTA-M2SG12EG19AP56DE148HR179
  • The table summarizes the amino acid exchanges within the TetR moiety of tTA discovered in the various tTA/rtTA alleles. The mutations were detected by DNA sequence analysis of alleles functionally identified in the yeast screening system.