Table 3

Medium with a high yeast content is required for efficient induction of female-specific lethality

Medium type*Yeast, g/literCornmeal, g/literNo. maleNo. female
High yeast10003301
Intermediate yeast6201031
Low yeast320201205
Intermediate yeast + cornmeal621072355
Low yeast + cornmeal32107170226
  • * All types of culture medium contained 100 g of sugar and 16 g of agar per liter. 

  • Males and females are the offspring of a cross between yp1-tTA line 19 with tetO-hid line 53 and thus carry one copy of each construct.