Table 1. cfu enumeration of wild-type and mutant M. tuberculosis in lungs of C57BL/6 mice
Lung cfu (log10)
M. tuberculosis H37Rv Day 1 Day 20 Day 50
Wild type 2.0 ± 0.05 6.5 ± 0.2 6.7 ± 0.08
cydC::aph 1.9 ± 0.03 6.5 ± 0.1 5.5 ± 0.2
cydC::aph::pHCYD 2.0 ± 0.1 6.1 ± 0.05 5.8 ± 0.05
  • C57BL/6 mice at 8-10 weeks of age were infected with ≈100 cfu of M. tuberculosis H37Rv WT, cydC::aph, and cydC::aph complemented with pHTB-CYD, which carries the M. tuberculosis cydABDC gene cluster on an integrative vector (Materials and Methods). Lungs were harvested at the indicated days postinfection, homogenized, serially diluted, and plated on enriched Middle-brook 7H11 agar plates for cfu enumeration. Data are expressed as means ± SD obtained from three to four mice per time point.