Table 2

Inhibition of medium alkalinization in response to elicitors in the presence of suramin or heparin

ElicitorPercentage of inhibition of medium alkalinization
+Suramin+Heparin
Systemin100  ± 01  ± 1
Chitosan73  ± 528  ± 8
pmg elicitor75  ± 523  ± 6
  • Cells of L. peruvianum were left untreated or supplied for 10 min with 1,000 μg/ml (=700 μM) suramin or 1,000 μg/ml heparin and then with 1 nM systemin, 50 μg/ml pmg elicitor, or 125 ng/ml chitosan. The pH change of the growth medium was determined 20 min after addition of elicitors and expressed as percentage of inhibition of medium alkalinization. The response to the elicitors alone was referred to as 0% inhibition.