Table 2

Expression of Tn5lacZ fusions in developing cells

StrainExpression β-galactosidase specific activity*Activity ratio§
StartC-dependFruiting bodiesPeripheral rods
pilA 0600  ± 52512  ± 431.2
Ω45212632  ± 27525  ± 791.2
Ω44553160  ± 16152  ± 181.0
Ω44694647  ± 58800  ± 850.8
Ω4414 (dev)6+470  ± 48107  ± 364.4
Ω75368+347  ± 5524  ± 214.4
Ω440311+323  ± 1466  ± 234.9
Ω445915+295  ± 1434  ± 118.7
Ω443520+170  ± 4048  ± 53.5
  • * β-galactosidase specific activity for separated fruiting body cells and peripheral rods carrying the indicated Tn5lacZ fusions (15) was determined after 24 h of development on TPM starvation agar. Mean specific activity ± SD were determined from three independent experiments. 

  • Hours after the initiation of development by starvation when each reporter starts its expression. 

  • + indicates that expression depends on C-signaling, and − indicates that it does not (33). 

  • § β-galactosidase specific activity in fruiting bodies divided by that in peripheral rods.