Table 3

Sporulation of dev mutants in submerged culture

StrainPercent wild-type sporulation
Morphological spores*Viable spores
DK1622100.0  ± (2.9)100.0  ± (3.4)
DK447312.6  ± (3.0)5.4  ± (2.1)
DK550828.0  ± (1.3)0.2  ± (0.2)
DK1123920.7  ± (1.1)1.3  ± (0.4)
  • Development in submerged culture for 5 days. Each assay was made three times. The mean values for three independent assays with the standard deviations in parentheses are shown. 

  • * Values were determined by counting the number of sonication-resistant, spherical-shaped cells in a Petroff-Hausser chamber observed by phase-control microscopy. 

  • Viable spore assay is described in Materials and Methods.