Table 2

Proteins comigrating in a single silver-stained spot on a 2D gel (spot 35 from Fig. 1B)

Gene name*Peptide sequences identifiedpI*Molecular mass, kDa
GDH1 (K)VIELGGTVVSLSDSK
(K)FIAEGSNMGSTPEAIAVFETAR
(R)EIGYLFGAYR5.5049.6
(K)VLPIVSVPER
RPT3 (R)ENAPSIIFIDEVDSIATK5.3247.9
SUB2 (R)DVQEIFR
(K)LTLHGLQQYYIK
(R)INLAINYDLTNEADQYLHR5.3650.3
(K)NKDTAPHIVVATPGR
(R)FLQNPLEIFVDDEAK
TIF3 (R)GSNFQGDGREDAPDLDWGAAR
(R)ADLVAVLK
(K)ITIPIETANANTIPLSELAHAK5.1748.5
(R)EREEVDIDWTAAR
(R)EREEPDIDWSAAR
VMA1 (K)VGHDNLVGEVIR5.0967.7
YFR044C (R)YPSLSIHGVEGAFSAQGAK
(K)LVYGVDPDFTR
(K)FISEQLSQSGFHDIK5.5452.9
(R)TELIHDGAYWVSDPFNAQFTAAK
(K)ILIDGIDEMVAPLTEK
  • * Gene names, predicted pI, and molecular mass values are from the Yeast Proteome Database (YPD) (13). 

  • Peptide sequences were identified automatically and verified manually by using sequest (12).