Table 3

Theoretical required total starting protein amounts for individual protein visualization by staining

Protein abundance, copies per cellSilver staining*Coomassie staining*
Protein amount, mgNumber of cellsProtein amount, mgNumber of cells
1020.0731.20 × 1092007.31.20 × 1011
1002.0071.20 × 108200.71.20 × 1010
1,0000.2011.20 × 10720.11.20 × 109
10,0000.0201.20 × 1062.01.20 × 108
100,0000.0021.20 × 1050.21.20 × 107
  • * Protein detection limits for silver and Coomassie staining were 1 and 100 ng, respectively. 

  • Soluble yeast protein was calculated based on 1 mg of yeast protein being derived from harvesting 6 × 107 cells. Calculations are based on a protein molecular mass of 50 kDa and 100% efficiencies of the procedures used.