Table 4

CBD inhibits Zymosan-induced release of reactive oxygen intermediates by mouse peritoneal granulocytes

Chemiluminescence peak
Granulocytes300
+ Zymosan1,868
+ Zymosan + CBD simultaneously1,024 (45%)
+ Zymosan + CBD pretreatment157 (92%)
  • Thioglycollate-elicited peritoneal granulocytes from C57BL/6 mice were stimulated with Zymosan in the absence or presence of CBD, 6 μg/ml. CBD was either added simultaneously with the Zymosan or the cells were pretreated with CBD for 1 h before stimulation. Production of reactive oxygen intermediates was measured by chemiluminescence. Percentage inhibition is shown in brackets. One representative experiment of four is shown.