Table 2

DNA sequence analysis of mini-MAR insertions

Clone no.Closest homologAccession no.blast score
 6Methanococcus jannaschii (MJ1507) Q58902 4e-24
 hypothetical orf
 7Pyrococcus horikoshii (PH0606) E71104 0.005
 Cdc47 homolog*
10 Methanosarcina mazei P35515 3e-47
DnaJ
13 Methanobacterium thermoautotrophicum O27585 2e-11
 lysS*
14 Anabaena variabilis U51863 5e-13
 VnfN
17 E. coli P27848 7e-04
 hypothetical orf yigL
18 M. mazei Y12024 le-15
orf46 S-layer protein
19Acetobacter plasmid pAH4JC23220.003
 hypothetical orf
  • The 20 mini-MAR insertions analyzed by DNA hybridization were cloned, and the junction sequences were determined as described in Fig. 3. The DNA sequence adjacent to the mini-MAR element (typically ca. 300–400 bp) were used to search the National Center for Biotechnology Information nonredundant protein database by using the BlastX algorithm on 4/19/2000. Eight of the 20 clones returned homologous sequences with P values < 0.005. The best score for each of these is listed above along with the associated SwissProt accession numbers and BlastX P values. 

  • * Disruption of these genes may reasonably be expected to be lethal; however, in these cases the insertion is adjacent to, but not within, the homologous gene.