Table 2

Horizontal spread and systemic infection of the P. aeruginosa ppk mutant in mouse tissues

StrainCFU/g tissue*
Horizontal spread within skin Systemic infection
8 h, Inoc. 8 h, Dist. 24 h, Inoc. 24 h, Dist. Spleen Liver
×104×104×107×107×103×102
PAO1 (WT)180  ± 7030  ± 7.6180  ± 32120  ± 28460  ± 65309  ± 61
PAOM5 (ppk)4.4  ± 1.80120  ± 5739  ± 102  ± 11  ± 0.4
  • * Mice were injected with ≈200 cells. CFUs were measured at 8 h and 24 h post-burn infection for horizontal spread within the skin and at 24 h for systemic infection in spleen and liver. Values represent averages ± SEM for 15 measurements in three separate experiments. Inoc., inoculation site; Dist., distant site (1.5 cm away).