Table 1

Ectopic expression of OmpT in place of OmpU diminishes V. cholerae toxT transcription and CT/TCP expression

Relevant genotype*PlasmidAggTCPCT§toxT∷lacZ% HEp-2 adherence
Wild type (toxR+)pWSK30+8.4  × 104101096  ± 59.1  (4.3)
ΔtoxR pWSK300<115  ± 13.4  (2.7)
ΔompU (toxR+) pWSK30+8.6  × 104880115  ± 610.0  (4.3)
ΔompU (toxR+) Up-U+4.4  × 104935111  ± 52.5  (2.9)
ΔompU (toxR+) Up-T7.7  × 1021062  ± 64.3  (6.1)
  • * Actual strains used (see Materials and Methods) O395, KKV61, KKV780, O395 toxT∷lacZ, KKV526, and KKV1089. 

  • Plasmids used (see Materials and Methods): Up-U, pKEK253; and Up-T, pKEK257. 

  • CTXΦKan transductants/ml/OD600

  • § ng/ml/OD600

  • Miller units based on triplicate samples. 

  • % adherent bacteria of total added; shown in parentheses are results of a second independent experiment.