Table 1

Poly(A) levels in various genetic backgrounds*

StrainGenotypePixels × 104
Hybridization probe
(dT)20(dT)30
MG1693rph-1§12  ± 23  ± 1
SK7988ΔpcnB rph-13  ± 10.4  ± 0.2
SK6699ΔpcnB pnp-7 rph-15  ± 10.6  ± 0.2
SK9166ΔpcnB pnp-7 ccacam rph-12  ± 10.6  ± 0.2
SK9161ΔpcnB rph-1/pKAK7 (pnp+)10  ± 21  ± 0.2
Control0.4  ± 0.20.4  ± 0.2
  • * Pixel values corresponding to the amount of 32P-labeled oligo(dT) probe that hybridized to total RNA isolated from each strain listed as determined with a Model 400 Molecular Dynamics PhosphorImager directly represent the amount of poly(A) present. Each value is the average of at least five or six independent experiments. 

  • Hybridization temperature of 30°C. 

  • Hybridization temperature of 50°C. 

  • § Jensen (28) has demonstrated that the progenitor of MG1693, MG1655, contains a frameshift mutation in rph

  • No RNA spotted on the hybridization membrane.