Table 1

Effect of disruptive agents on tip links

AgentScore
1 mM LaCl3−  (4)
5 mM LaCl3−  (2)
1 mM TbCl3+/−  (3)
5 mM TbCl3−  (1)
20 mM BaCl2+/−  (3)
10 mM sodium periodate+/−  (8)
25 mM mannose-6-phosphate+/−  (4)
10 mM acetylneuraminic acid+/−  (2)
1 mg/ml elastase, 200 units/ml N-glycosidase F+/−  (3)
1 mg/ml elastase, 10 units/ml chondroitinase ABC, 200 units/ml N-glycosidase F−  (5)
1 mg/ml chymotrypsin, 200 units/ml N-glycosidase F+/−  (3)
1 mg/ml chymotrypsin, 10 units/ml chondroitinase ABC, 200 units/ml N-glycosidase F−  (5)
  • Basilar papillae were treated for 10 min with the indicated reagents. For combinations of glycosidases (N-glycosidase F and chondroitinase) and proteases, papillae were first treated with glycosidases for 30 min, followed by a wash and a 10-min protease treatment. Scores: (+), 50–100% number of tip links as control; (+/−), 15–50% of control; (−), 0–15% of control; number of papillae examined in parentheses. The following were scored (+) with one or two papillae each: 1 mM BaCl2, 1 mM CoCl2, 5 mM MnCl2, 10 mM galactose, 10 mM mannose, 10 mM lactose, 10 mM fucose, 10 mM mannose-1-phosphate, 25 mM fucose-1-phosphate, 10 mM N-acetyl-d-galactosamine, 10 mM N-acetyl-d-glucosamine, 1 mM methyl-α-d-mannopyranoside, 5 mM heparin, 1 mg/ml chondroitin sulfate, 100 μg/ml fucoidan, 200 units/ml N-glycosidase F, and 10 units/ml chondroitinase ABC. The following were scored (+) with one or two papillae each at 0.5 mg/ml and 1 mg/ml: trypsin, chymotrypsin, papain, bromelain, ficin, protease K, S. aureus V8 protease, and elastase.