Table 2

Effects of stimuli on the filamentous morphology

Stimulus added*% yf% ffyf radius§
 Glu (1 M)>95<520
 Glu (0.25 M)>95<515
 Glu (0.06 M)>95<54
 Sucrose (1 M)>95<512
 Mannose (1 M)>95<511
 Glycogen (100%)<5>950
 Glycerol (100%)<5>950
 Glu (1 M) + 0.1% SDS>95<520
 Glu (1 M) + 1 M NaCl>95<520
 YPD>95<520
 YP<5>950
 Peptone (10%)<5>950
 Yeast extract (10%)<5>950
Stimulus removed
 Nitrogen9010NA
 Amino acids>95<5NA
 Uracil>95<5NA
  • * Stimulus (10 μl) was spotted onto discs and placed on SC medium; Glu, glucose. 

  • % yf denotes round cells and axial budding for >200 microcolonies adjacent to the disc. 

  • % ff denotes elongated cells and unipolar budding for >200 microcolonies adjacent to the disc. 

  • § Distance (in mm) from the center of a disc to the point at which only ff colonies were observed. 

  • Some unipolar budding (<20%) was observed. 

  • SCD medium lacking the noted ingredient; for nitrogen, SLAHD medium was prepared as described in Materials and Methods; NA, not applicable.