Table 2

Correlation of plasmid content with infectivity in Bb B31

ClonePlasmid content*
Infectivity associated Present in all clones Not infectivity associated
lp25lp28-1cp26cp32-1cp32-2,7cp32-4cp32-6cp32-8cp32-9lp17lp28-3lp36lp38lp54cp9cp32-3lp21lp28-2lp28-4lp56
High-infectivity phenotype
5A1+++++++++++++++++++
5A3+++++++++++++++++
5A4++++++++++++++++++++
5A6++++++++++++++++++
5A9+++++++++++++++++++
5A11++++++++++++++++++++
5A15++++++++++++++++++
5A16+++++++++++++++++++
5A18++++++++++++++++++
5A19++++++++++++++++++++
Intermediate-infectivity phenotype
5A2+++++++++++++++++
5A8+++++++++++++++++++
Low-infectivity phenotype
5A5++++++++++++++++++
5A7++++++++++++++++++
5A10++++++++++++++++++
5A12+++++++++++++++++
5A13+++++++++++++++++++
5A14++++++++++++++++++
5A17++++++++++++++++++
  • * As determined by PCR amplification (Fig. 1) and confirmed by Southern hybridization for lp25 and lp28-1 (Fig. 2). 

  • Infectivity phenotype determined by needle inoculation of C3H/HeN mice (Table 1).