Table 1

Oligonucleotides used in this study

DesignationSequence
ychBvo 5′-GAGGAGAAATTAACCATGCGGACACAGTGGCCC-3′
ychBhi 5′-GTCACCGAACTGCAGCTTGCCCG-3′
kEcoRI 5′-ACACAGAATTCATTAAAGAGGAGAAATTAACCATG-3′