Table 1

Amounts of glycolytic enzymes compared to glycolytic flux for bloodstream-form T. brucei and S. cerevisiae

Glycolytic enzyme% of total protein
T. bruceiS. cerevisiae
Hexokinase0.250.08
Glucose-6-phosphate isomerase0.150.32
Phosphofructokinase0.390.23
Aldolase1.20.64
Triose-phosphate isomerase0.040.07
Glyceraldehyde-3-phosphate dehydrogenase0.502.5
Phosphoglycerate kinase0.160.53
Glycerol-3-phosphate dehydrogenase0.25
Alcohol dehydrogenase0.59
Total 2.9 5.0
μmol⋅min−1⋅(mg protein)−1
Glycolytic flux0.150.33
  • Data for T. brucei are from refs. 18 and 22. Only the glycosomal enzymes of T. brucei were taken into account. For yeast, alcohol dehydrogenase was taken rather than glycerol-3-phosphate dehydrogenase, since it fulfills the same function (glycolytic redox balance). Glycolytic flux was defined as glucose consumption rate, which equals half the aerobic pyruvate production rate (22) in the case of T. brucei and half the anaerobic ethanol production rate in the case of S. cerevisiae. When T. brucei is compared to S. cerevisiae, the glycolytic flux is almost proportional to the amount of glycolytic protein.