Table 1

Kinetic parameters for serine-dependent ATP/[32P]PPi exchange

Constructkcat, min−1Km, mMkcat/Km min−1 mM−1
EntF full length670  ± 200.6  ± 0.031,120
EntF C-A 1-974-His6990  ± 8014  ± 270
EntF C-A 1-974340  ± 1022  ± 315
EntF C-A 1-100516  ± 3NDND
  • ND, not determined.