Table 3

Pyrophosphate release rates

Adenylation constructAdded carrier proteinPPi release rate, min-1Acylation rate, min−1
Salicylate dependentSerine dependentSalicylationSerylation
EntE 9 ±1
EntEEntB ArCP95 ± 5130
EntESrfB1 PCP 9 ± 10.5
EntF C-A2.3 ± 0.4
EntF C-ASrfB1 PCP2.0 ± 0.30.5
EntF C-AYbt PCP12.2 ± 0.30.01