Table 1

Type IV pili-mediated twitching motility of P. aeruginosa strains

StrainRelevant genotypeTwitch area, % WT ± SEM*
PAO1WT100  ± 4
PAOM5Δppk63  ± 2
PAO-DΔfliD93  ± 4
PAO-NPΔpilA  0
PAO1 (pMMB66HE)WT + vector100  ± 5
PAOM5 (pMMB66HE)Δppk + vector55  ± 2
PAOM5 (pHEPAK11)Δppk + PPK+++97  ± 4
  • * Twitch area was measured after a 24-h incubation at 37°C on LB agar (1%, wt/vol) plates. The standard error of the mean (SEM) equals σn−1Embedded Image, where n = 6.