Table 1

The sequences of the wild-type (WT) and mutated LPK ChREs

OligonucleotideSequence
WT 5′-GGGCGCACGGGGCACTCCCGTGGTTCC-3′
3′-CCCGCGTGCCCCGTGAGGGCACCAAGG-5′
M1 5′-GGGCGaACGGGGCACTCCCGTtGTTCC-3′
3′-CCCGCtTGCCCCGTGAGGGCAaCAAGG-5′
M2 5′-GGGCGCcCGGGGCACTCCCGgGGTTCC-3′
3′-CCCGCGgGCCCCGTGAGGGCcCCAAGG-5′
M3 5′-GGGCGCAaGGGGCACTCCCtTGGTTCC-3′
3′-CCCGCGTtCCCCGTGAGGGaACCAAGG-5′
M4 5′-GGGCGCACtGGGCACTCCaGTGGTTCC-3′
3′-CCCGCGTGaCCCGTGAGGtCACCAAGG-5′
M5 5′-GGGCGCACGtGGCACTCaCGTGGTTCC-3′
3′-CCCGCGTGCaCCGTGAGtGCACCAAGG-5′
M6 5′-GGGCGCACGGtGCACTaCCGTGGTTCC-3′
3′-CCCGCGTGCCaCGTGAtGGCACCAAGG-5′
M3/5 5′-GGGCGCAtGcGGCACTCgCaTGGTTCC-3′
3′-CCCGCGTaCgCCGTGAGcGtACCAAGG-5′
S−1 5′-GGGCGCACGGGGCCTCCCGTGGTTCCT-3′
3′-CCCGCGTGCCCCGGAGGGCACCAAGGA-5′
S+1 5′-GGGCGCACGGGGCACTTCCCGTGGTTCC-3′
3′-CCCGCGTGCCCCGTGAAGGGCACCAAGG-5′
  • The E-boxes are in boldface type. Mutations within the E-box are indicated by lowercase letters. S−1 and S+1 indicate the deletion or addition, respectively, of one base between the two E-boxes.