Table 1

Main biochemical parameters in plasma and urine and renal morphometric data 12 weeks after induction of diabetes

One-copy Two-copy Three-copy P values*
CT (n = 8)DM (n = 11)CT (n = 8)DM (n = 6)CT (n = 7)DM (n = 7)GenotypeDiabetesInteraction
Body weight, g22.5  ± 0.518.3  ± 1.121.8  ± 0.521.0  ± 0.622.7  ± 0.720.3  ± 0.30.27<0.0010.08
Plasma
 Glucose, mmol/l7.2  ± 0.327.5  ± 1.27.4  ± 0.323.5  ± 1.06.9  ± 0.324.4  ± 1.60.18<0.00010.07
 Hematocrit, %43.1  ± 0.844.9  ± 1.843.2  ± 0.844.5  ± 1.743  ± 0.642.9  ± 2.30.820.250.65
 Creatinine, μmol/l13.1  ± 1.116.3  ± 1.311.5  ± 1.115.5  ± 0.814.7  ± 1.416.7  ± 0.80.21<0.010.71
 PRC, μgAI/ml/h1.29  ± 0.140.94  ± 0.170.82  ± 0.130.78  ± 0.180.86  ± 0.120.59  ± 0.110.030.070.57
Urine
 Urine volume, ml/24 h0.5  ± 0.119.3  ± 3.50.5  ± 0.113.0  ± 1.50.7  ± 0.119.4  ± 2.70.14<0.00010.18
 Creatinine clearance, μl/min127  ± 32224  ± 33142  ± 26187  ± 16139  ± 22237  ± 210.72<0.0010.59
 UKE, μgBK/min/24 h urine1.49  ± 0.391.48  ± 0.231.59  ± 0.312.10  ± 0.392.94  ± 0.763.31  ± 0.32<0.010.060.13
Kidney weight
 Absolute, mg120  ± 4164  ± 7112  ± 3140  ± 7124  ± 13176  ± 3<0.01<0.00010.25
 Relative, mg/g BW5.4  ± 0.19.2  ± 0.55.1  ± 0.16.7  ± 0.35.4  ± 0.68.7  ± 0.1<0.01<0.00010.16
Morphometric data
 Glomerular tuft surface area, μm22226  ± 582785  ± 1032284  ± 672553  ± 802077  ± 712555  ± 930.11<0.00010.08
 Mesangial surface area, μm2531  ± 33571  ± 32598  ± 19571  ± 26585  ± 27630  ± 310.200.630.23
 Bowman's capsule surface area, μm2207  ± 8304  ± 41292  ± 9336  ± 14291  ± 14377  ± 200.01<0.00010.56
 Mean thickness of Bowman's capsule, μm2.56  ± 0.122.01  ± 0.222.07  ± 0.071.70  ± 0.042.03  ± 0.101.69  ± 0.090.03<0.00010.9
  • CT, control groups; DM, diabetic groups; Creat, creatinine; PRC, plasma renin concentration; UKE, urinary kallikrein excretion. Results are means ± SEM; AI, angiotensin I; BK, bradykinin; BW, body weight. 

  • * Two-way ANOVA.