Table 1

Bacterial strains and plasmids used

Strain or plasmidGenotypeSource/ref.
P. aeruginosa
 PAO1Wild-type prototroph30
 PAOR3qscR mutant of PAO1, GmRThis study
E. coli
 DH5αF φ80dlacZΔM15Δ(lacZYA-argF)U169endA1 recA1 hsdR17 deoR gyrA96 thi-1 relA1 supE4431
 S17-1thi pro hsdR recA RP4-2 (Tet∷Mu) (Km∷Tn7)32
 CM830E. coli K-12 derivative
Plasmids
 pGmΩ1Broad-host-range vector containing the aacC1 gene, GmR33
 pCR2.1TA cloning vector, ApRInvitrogen
 pEX1.8Broad-host-range expression vector, ApR34
 pQF50Broad-host-range lacZ transcriptional fusion vector, ApR35
 pMP7E. coli cloning vector, (ColE1) ori, ApR36
 pKL13pMP7 containing 3.1 kb qscR fragment, ApRThis study
 pKL14pKL13 with 1.6 kb aacC1 interrupting qscR ApRGmRThis study
 pKV69Vector carrying mobRP4 CmRTcRK. Visick, Loyola Univ., Chicago
 pMW16pKL14 containing 1.6 kb mobRP4 from pKV69, ApRGmRCmRThis study
 pKL8pCR2.1 containing 714 bp qscR fragment, ApRThis study
 pKL9pEX1.8 containing ptac-qscR, ApRThis study
 pMW303Broad-host-range phzABC-lacZ reporter, ApR15
 pMW301Broad-host-range hcnAB-lacZ reporter, ApR15
 pSC11Broad-host-range lasI-lacZ reporter, ApRThis study
 pSC10Broad-host-range lasR-lacZ reporter, ApRThis study
 pMW305Broad-host-range rhlI-lacZ reporter, GmR, SpR, SmR15
 pMW304Broad-host-range rhlR-lacZ reporter, GmR, SpR, SmR15
 pMW105BBroad-host-range qsc105-lacZ reporter, ApRThis study