Table 2

MTCYP51 active-site residues

MTCYP51PlantMammalsFungi
Y76 Y Y Y
F78FRH(7), V(3), K(1), N(1)
M79NLL
F83 F F F
F89 F Y Y
K97QKQ(11), H(1)
M99RM(2), I(2)K
H101FM(4), V(3), A(3), I(2)
S252AGA(6), G(5), T(1)
F255FLM
H259 H H H
T260 T T T(7), S(5)
L321 L I L(6), I(6)
I323MT(3), I(1)S(1), T(1)
M433 M M M(7), L(4)
V434VIV(6), F(5)
  • Altogether, three plant, four animal, and 12 fungi CYP51 sequences were analyzed. Alignment was performed using bcm search launcher (34). Bold letters indicate residues that display significant conservation through evolution. Parentheses shows the numbers of species with indicated substitution.