Table 1

Identification of C. crescentus proteins with differential cell cycle expression profiles

ORFCLFunction
Amino acid metabolism
 03246*16Tetrahydropteroyltriglutamate methyltransferase (Met)
 04899*3Glutamyl-tRNA(Gln)-amidotransferase subunit A (Glu)
 0597419Indole-3-glycerol-phosphate synthase (Trp)
 0664319Acetylornithine aminotransferase (Arg)
Carbohydrate metabolism
 033101Acetyl-CoA-acetyltransferase
 038617 β-d-glucoside glucohydrolase
 06912*7UDP-glucose-4-epimerase
Cofactor metabolism
 032066GTP cyclohydrolase I (tetrahydrofolate synthesis)
 04038*6Riboflavin specific deaminase
 04041*6Riboflavin synthase α-chain
 040426GTP cyclohydrolase II (riboflavin synthesis)
 04043*6Riboflavin synthase β-chain
Lipid metabolism
 023023Enoyl-CoA-hydratase
 024367Fatty acid oxidation complex α-subunit
Energy metabolism
 0168622 ATP-synthase-α-subunit
 0211022Aconitase
 06056*23NADH dehydrogenase I chain G
 0625522Pyruvate kinase
Redox reactions
 0053221Thioredoxin reductase
 009523Glutathione-S-transferase
 01378*1NADPH-dependent quinone oxidoreductase
 02058*11Glutathione-S-transferase
 02406*1NAD(P)H Nitroreductase
 02506*1Thioredoxin
RNA and protein synthesis
 0259523Ribonuclease PH
 035531450S Ribosomal protein L1
 044209Peptide chain release factor 3
 04755650S Ribosomal protein L4
 05538*2350S Ribosomal protein L9
Protein folding and degradation
 004741, 22Aminopeptidase
 00833*21Peptidase
 0127019Peptidase
 01800*1, 7Endopeptidase
 019441Zinc-metalloprotease
 021432 Prolyl-endopeptidase
 021453Prolyl-endopeptidase
 02174*7Peptidyl-dipeptidase
 03639*8Chaperone GroEL
 03641*8Chaperone GroES
 06085*12Protease ClpP
 06128*7Dipeptidyl-peptidase
 07050*23Amidohydrolase
Cell envelope synthesis and structure
 017712Penicillin binding protein
 0509512-Dehydro-3-deoxyphosphooctonate aldolase
Transport protein
 01077*13TonB-dependent iron uptake receptor
 01388*23ABC transporter ATP binding protein
 017907TonB-dependent iron uptake receptor
 056912TonB-dependent receptor
Motility and chemotaxis
 03149*13 Chemotaxis protein CheYI
 03156*11Chemotaxis protein CheR
 03157*13Chemotaxis protein CheB
 03161*13 Chemotaxis protein CheD
 05156*13 Flagellin FljL
 05158*4 Flagellin FljK
Cell division
 07201*20 Cell division protein FtsZ
DNA metabolism
 0219723dUTP nucleotidohydrolase
 03051*13 DNA methyltransferase CcrM
 05175*21Single strand binding protein SSB
Regulatory protein
 00839*15 Cell cycle transcriptional regulator CtrA
 01137*23Transcriptional regulator of AsnC/Lrp family
 0240022Ferric uptake regulation protein Fur
 02881*1Phosphate transport regulator PhoU
 05154*10Regulator of flagellin expression FlbT
 05981*17SOS response repressor LexA
 06096*5Nitrogen regulatory protein PII
 07059*12Response regulator PleD
 07061*13Response regulator DivK
Unknown function
 01570*1Conserved unknown
 02168*21Unknown
 02609*21Unknown
 03189*12Conserved unknown
 03651*10 Unknown
 0391022Conserved unknown
 0444921Unknown
 0547510Unknown
 05886*12Conserved unknown
 05929*7Conserved unknown
 06393*1Conserved unknown
 06615*2Conserved unknown
 0709412Unknown
 0719822Conserved unknown
  • ORF, Caulobacter genome ORF number (see Materials and Methods); *, similar; †, no or inverse cell cycle oscillations were detected for the corresponding mRNAs in ref. 4; CL, cluster number of coexpression groups (Fig. 2); Function, putative function determined by blast homology searches. Proteins with a half life shorter than one cell cycle are shown in bold.