Table 3

Inhibition of cell–cell fusion by X5 and b12

EnvelopeCladeAntibody
X5b12
UG037.8A2924
RW020.5A5658
US715.6B3528
HT593.1B2039
US005.11B2447
89.6B100100
BR025.9C6222
ZR001.3D4439
TH022.4E1734
BR029.2F4224
BR019.4F/B6263
UG975.1G2541
  • 105 293 cells, transfected with plasmids encoding various HIV Envs under the control of T7 promoter and infected with recombinant vaccinia virus encoding T7 polymerase gene, were preincubated with X5 or IgG b12 at 100 μg/ml for 30 min at 37°C, and then mixed with 105 CEM-CCR5 cells infected with recombinant vaccinia virus encoding β-galactosidase gene. The extent of cell fusion was quantified colorimetrically 2 h after mixing the cells. The data are averages of duplicate samples and presented as % of fusion inhibition.