Table 1

[Het-s] induction after biolistic introduction of the recombinant HET-s into [Het-s*] (prion-free) strains

ProteinAmount of protein[Het-s] mycelia% of prion induction
Controls
 No shot1/1261
 No protein12/4163
 BSA4.51/323
Aggregated HET-s (converted)4215/21699
Aggregated HET-s (spontaneous)659/6887
154/7077
0.346/6472
0.142/6070
0.0424/3275
0.018/3225
0.0044/686
Freshly renatured HET-s3.516/1819
Proteinase K-digested aggregates256/6487
Amorphous HET-s aggregates
 TCA-precipited43/565
 Heat-denatured42/524
Aβ40 peptide amyloids1.42/484
  • The first column gives the protein used in biolistic. The second column gives the amount of protein in micrograms per mycelium. The third column gives the number of strains that acquired the [Het-s] prion over the total number of strains. The last column gives the corresponding percentage of [Het-s] induction. TCA, trichoracetic acid. 

  • Amount of HET-s prior to digestion.