Table 1

A summary of the genes significantly* up- or down-regulated by mutation in M. tuberculosis sigH at four different phases of growth in vitro

M. tuberculosis genes in the sigH mutant
OD600Down-regulated by 2.0-fold or moreUp-regulated by 2.0-fold or more
0.315 genes including  MT4032 (thioredoxin reductase); MT4033, MT1516, and MT1517 (thioredoxins); MT2063 (ferredoxin); MT2468 and MT2469 (sulfate ABC transporters); MT1163 (citrate synthase); MT2541 (conserved hypothetical protein containing a glutaredoxin active site); MT1872 (small basic protein); MT1377 (cysteine synthase); MT0040 (AMP-binding family protein); MT1924 (conserved hypothetical protein); MT1378 (rhomboid family protein); and MT3320 (sigH)3 genes including  MT2310 (transcriptional regulator); MT0941 (PE family protein); and MT3850 (hypothetical protein)
0.623 genes including  MT3320 (sigH); MT1259 (sigE); MT2783 (sigB); MT4032 (thioredoxin reductase); MT4033 (thioredoxin); MT3938; MT1297 (multidrug resistance efflux protein); and MT2541 (conserved hypothetical protein containing a glutaredoxin active site)9 genes including  MT2310 (transcriptional regulator); MT0941 (PE family protein); MT2438 (hypothetical protein); MT0292 (PPE family protein); MT2050 (transcriptional regulator, arsR family); MT2027 (DNA-binding protein, copG family); MT2448 (peptide synthetase family); MT0146 (hypothetical protein); and MT0340 (P450 heme thiolate protein)
1.2121 genes including  MT3320 (sigH); MT1259 (sigE); MT2783 (sigB); MT4032 (thioredoxin reductase); MT4033 (thioredoxin); MT3938; MT1297 (multidrug resistance efflux protein); MT2541 (conserved hypothetical protein containing a glutaredoxin active site); and MT0397 (clpB)60 genes including  MT2310 (transcriptional regulator); MT0941 (PE family protein); MT2438 (hypothetical protein); MT0292 (PPE family protein); MT2050 (transcriptional regulator, arsR family); MT2027 (DNA-binding protein, copG family); MT2448 (peptide synthetase family); MT0146 (hypothetical protein); MT0340 (P450 heme thiolate protein); and MT2446 (hypothetical protein)
>2.0164 genes including  sigH; sigE; sigB; MT4033; MT4032; MT3938 (tetR transcriptional regulator); MT3555 (serine protease); MT2394 (malate oxidoreductase); MT2303 (malonyl-CoA acyl carrier protein); MT2150 (Xaa-Pro dipeptidase); MT2063 (ferredoxin); MT1896 and 1897 (urease subunits); MT1604–1606 (fumarate reductase operon); MT1516–1517 (thioredoxins); MT1297 (multidrug resistance efflux protein); MT1377 (cysteine synthase); MT1163 (citrate synthase); MT0631 (tcrA response regulator); MT0397 (clpB, ATP-binding subunit of clp protease); and MT2541 (conserved hypothetical protein containing a glutaredoxin active site)71 genes including  MT2310 (transcriptional regulator); MT0941 (PE family protein); MT2438 (hypothetical protein); MT0292 (PPE family protein); MT2050 (transcriptional regulator, arsR family), MT2027 (DNA-binding protein, copG family), MT2448 (peptide synthetase family); MT0146 (hypothetical protein); MT0340 (P450 heme thiolate protein); and MT2446 (hypothetical protein)
  • The complete list of genes significantly down-regulated by M. tuberculosis sigH mutation at OD600 of 1.2 is available as supporting information on the PNAS web site, www.pnas.org

  • * Significant modulation was defined as a wild-type to mutant intensity ratio of ≥2.0 or ≤0.5 in at least 75% of the evaluable spots.