Table 1

Protocol for the purification of the systemin receptor from L. peruvianum

Purification stepTotal protein, mgTotal activity, cpmRecovery, % of startSpecific activity, cpm/mg × 10−6Fold purification (over previous step)Fold purification (total)
Crude extract2,73051,000,0001000.018721
Membrane isolation1,06445,500,00089.20.04282.292.29
Preparative SDS/PAGE1.628,000,00015.74.94115.4264
Con A affinity0.0132,000,0003.915431.28,235