Table 1.

The EC50 values (in nM) of various peptides for HEK-MrgA1 and HEK-MrgC11 cells using FLEXstation assay

PeptidesSequencesMrgC11MrgA1
AnthoRF- amide pEGRFa    16  ± 6Inactive
AF-2 KHEYLRFa    130  ± 24Inactive
ACEP-1 SGQSWRPQGRFa    46  ± 12Inactive
FLRF-amide FLRFa    157  ± 12402  ± 21
FMRF-amide FMRFa    114  ± 32420  ± 71
FMRF-OH FMRF     544  ± 1178,204  ± 458
Met-ENK-RF- amide YGGFMRFa    133  ± 205,252  ± 1,280
Met-Enk-RF YGGFMRF     545  ± 19Inactive
γ1-MSH YVMGHFRWDRFa    17  ± 3Inactive
γ2-MSH YVMGHFRWDRFG    11  ± 5Inactive
BAM3200 YGGFMRRVGRPEWWMDYQKRYGGFL 300  ± 124>10,000
BAM-22P YGGFMRRVGRPEWWMDYQKRYG    26  ± 102,542  ± 654
BAM-15 VGRPEWWMDYQKRYG    53  ± 223,326  ± 1,866
BAM-15- amide VGRPEWWMDYQKRYa    479  ± 148,773  ± 493
Dynnorphin- 14 IRPKLKWDNQKRYG    22  ± 1Inactive
PrRP-20 TPDINPAWYTGRGIRPVGRFa    144  ± 18Inactive
Kiss(107–121) KDLPNYNWNSFGLRFa    102  ± 24Inactive
Kiss(112–121) YNWNSFGLRFa    50  ± 4Inactive
PQRF-amide PQRFa    126  ± 28>10,000
NPFF FLFQPQRFa    54  ± 52,145  ± 245
NPAF AGEGLNSQFWSLAAPQRFa    282  ± 30Inactive
RFRP-1 MPHSFANLPLRFa    1,245  ± 112Inactive
RFRP-3 VPNLPQRFa    113  ± 5Inactive
NPY YPSKPEDMARYYSALRHYINLITRQRYa    237  ± 303,486  ± 986
  • Data represent means ± SEM from triplicate independent determinations. Inactive, no activation was detected at concentrations up to 10 μM.