Table 2

Summary of in vivo muscle regeneration experiments

Injected sampleNo. of injected cells × 103No. of injected musclesEngraftment*
ModerateExtensive
Unsorted43–100532
Sca-1+44–84621
Sca-1−80–140605
Sca-1+/CD45+17–78912
Sca-1+/CD45−3.4–12913
C57/mlacZ CD45+124430
 74210
 40440
C57/MlacZ CD45− 74202
 15421
PB18–195300
  •  All experiments were performed with Rosa26-derived muscle cells except those noted as C57/MlacZ

  • * Engraftment is defined as a fiber staining either solid or punctate blue throughout and identifiable in multiple serial sections of TA muscle. 

  • Moderate engraftment is defined as faint β-galactosidase staining in one to two blue fibers identified or unorganized aligned blue cells. 

  • Extensive engraftment is defined as more than three blue fibers identified or dark blue nuclei clearly incorporated into a regenerating fiber.