Table 1

Strains and plasmids used in this study

PropertyReference
Strains
S. aureus NewmanHuman clinical isolate, wild-type srtA and srtB 21
S. aureus SKM7Isogenic Newman variant, Δ(srtB)This study
S. aureus RN4220Restriction deficient laboratory strain, wild-type srtA and srtB 20
S. aureus SKM1Isogenic variant of RN4220, Δ(srtA) 17
S. aureus SKM5Isogenic variant of RN4220, Δ(srtB)This study
S. aureus SKM15Isogenic variant of RN4220, Δ(fur)This study
S. aureus SKM16Isogenic variant of RN4220, Δ(fur, srtB)This study
E. coli BL21 (DE3)E. coli B strain, λ lysogen, T7 expression 23
Plasmid
 pSM74seb promoter and orf fused to 5′ of isdC sorting signalThis study
 pSM75pSM74 plus srtB expressed from the srtA promoterThis study
 pSM79pSeb-Spa plus srtB expressed from the srtA promoterThis study
 pSM80seb promoter fused isdC orf carrying a FLAG epitope tagThis study
 pSM83pSM80 plus srtB expressed from the srtA promoterThis study
 pSM84pET16a derivative, srtBΔN expressed from the T7 promoterThis study
 pSeb-Spaseb promoter and orf fused to 5′ end of spa sorting signal 10
 pSeb-FnbAseb promoter and orf fused to 5′ end of fnbA sorting signal 10
 pSeb-FnbBseb promoter and orf fused to 5′ end of fnbB sorting signalThis study
 pSeb-ClfAseb promoter and orf fused to 5′ end of clfA sorting signal 17
 pSeb-ClfBseb promoter and orf fused to 5′ end of clfB sorting signalThis study
 pSeb-SdrDseb promoter and orf fused to 5′ end of sdrD sorting signalThis study
 pSeb-SdrEseb promoter and orf fused to 5′ end of sdrE sorting signalThis study
 pSeb-SasAseb promoter and orf fused to 5′ end of sasA sorting signalThis study
 pSeb-SasBseb promoter and orf fused to 5′ end of sasB sorting signalThis study
 pSeb-SasCseb promoter and orf fused to 5′ end of sasC sorting signalThis study
 pSeb-SasEseb promoter and orf fused to 5′ end of sasE sorting signalThis study
 pSeb-SasFseb promoter and orf fused to 5′ end of sasF sorting signalThis study
 pSeb-SasGseb promoter and orf fused to 5′ end of sasG sorting signalThis study
 pSeb-SasHseb promoter and orf fused to 5′ end of sasH sorting signalThis study
 pSeb-SasJseb promoter and orf fused to 5′ end of sasJ sorting signalThis study