Table 1

AdhE activity levels of different strains grown aerobically or anaerobically in glucose medium in the presence or absence of IPTG to induce DnaK

StrainDerived fromRelevant genotype*Ethanol oxidoreductase specific activity, nmol/min per mg
Aerobic Anaerobic
−IPTG+IPTG−IPTG+IPTG
ECL4000MC4100araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR attB∷Φ(adhE-lacZ)86  ± 1098  ± 12660  ± 7700  ± 20
JMH800ECL4000ΔdnaK adhE+31  ± 628  ± 08640  ± 90700  ± 10
JMH801ECL4000PIPTGdnaKJ adhE+29  ± 1097  ± 10711  ± 70900  ± 10
JE52ECL4000 dnaK + adhE A267T/E568K 580  ± 90550  ± 701,600  ± 1301,800  ± 200
JMH802JE52ΔdnaK adhEA267T/E568K49  ± 1250  ± 161,600  ± 1801,750  ± 300
JMH803JE52PIPTGdnaKJ adhEA267T/E568K120  ± 11670  ± 1001,900  ± 2601,900  ± 200
  • * For detailed description of dnaK alleles see Materials and Methods.