Table 1

Bacterial strains and plasmids used in this study

NameRelevant characteristicsSource or ref. no.
B. fragilis
 TM4000Wild-type, RifR, TpS 23
 ADB77TM4000 ΔthyA1, TpRLab stock
 ADB237ADB77 acnA∷pADB237, TetRThis work
 ADB245ADB77 ΔacnA245This work
E. coli
 DH5αΔlacZ M15 14
 DW1030Host strain for pRK2317 42
Plasmids
 pCR2.1TOPOTA cloning vector, lacZα, AmpRInvitrogen
 pRK231.7RP4 derivative, TetR, Tra+, AmpS 15
 pYT102p15A ori, CmR, RP4 oriT; B. fragilis suicide vector, thyA+, TetR 17
 pADB237pYT102 with acnA internal fragmentThis work
 pADB245pYT102 ΔacnA245This work
 pADB250pCR2.1TOPO containing acnAThis work