Table 1

NMR structure statistics

NMR constraints
TAZ1Hif-1α
Individual domains
 Unambiguous distance restraints
  Intraresidue448145
  Sequential + medium range602520
  Long range (|ij| > 4)14524
 Ambiguous distance restraints414
 Torsion angle restraints79 φ, 58 ψ, 45 χ129 φ, 16 ψ, 23 χ1
TAZ1/Hif-1α complex
 Intermolecular distance restraints92
 Ambiguous restraints28
Structure statistics (20 structures)
Violation statistics
 NOE violations >0.1 Å/structure8  ± 2
 Maximum NOE violation/structure, Å0.17  ± 0.05
 Dihedral constraint violations/structure0.7  ± 0.7
 Maximum torsion angle violation/structure, °4  ± 4
Energies
 Mean GB* constraint violation energy, kcal/mol8  ± 1
 Mean GB AMBER energy, kcal/mol−8,496  ± 20
Mean deviations from ideal covalent geometry
 Bond lengths, Å0.0061  ± 0.0002
 Bond angles, °2.40  ± 0.03
PROCHECK statistics
 Residues in most favored regions87.2     
 Residues in allowed regions12.0     
 Residues in generously allowed regions 0.6     
 Residues in disallowed regions 0.1     
RMS deviations from the average structure
 TAZ1/HIF-1α complex (residues 345–435; 792–825)
  Backbone atoms (N, Cα, C′), Å 0.91
  All heavy atoms, Å 1.39
 Helices only
  Backbone atoms (N, Cα, C′), Å 0.45
  All heavy atoms, Å 0.91
  • * GB, generalized Born. 

  • RMS, root-mean-square.